ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship of Epstein-Barr virus (EBV) specific antibodies detection and the diagnoses of infectious mononucleosis (IM) caused by EBV.</p><p><b>METHODS</b>Single serum samples were collected from 220 inpatients with the diagnosis of IM between January 2005 and December 2006 in Beijing Children's hospital. The samples were detected for EBV-CA-IgM, EBV-CA-IgG, EBV-EA-IgG, EBV-NA-IgG and the avidity of EBV-CA-IgG by indirect immunofluorescent assay.</p><p><b>RESULTS</b>The positive rate of EBV-CA-IgG, EBV-CA-IgM, EBV-EA-IgG and EBV-NA-IgG were 100%, 95.9%, 79.5% and 4.1% respectively. Low-avidity EBV-CA-IgG was detected in 204 patients (92.7%) with positive EBV-CA-IgM and EBV-CA-IgG,negative EBV-NA-IgG, low-avidity EBV-CA-IgG were the main pattern of EBV antibody in IM patients (84.6%).</p><p><b>CONCLUSION</b>The existence of EBV specific antibody (CA-IgG, CA-IgM, EA-IgG, NA-IgG and avidity of CA-IgG) could add more information to identify the stage of EBV infection so as to provide more reliable serological evidence for the diagnosis of IM.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Infant , Male , Antibodies, Viral , Blood , Antibody Affinity , Epstein-Barr Virus Infections , Diagnosis , Allergy and Immunology , Fluorescent Antibody Technique, Indirect , Herpesvirus 4, Human , Immunoglobulin G , Blood , Infectious Mononucleosis , Diagnosis , Allergy and Immunology , VirologyABSTRACT
<p><b>OBJECTIVE</b>To investigate genotype of wild-type measles viruses circulated in Beijing in 2003.</p><p><b>METHODS</b>Throat swabs specimens were collected from patients seen during an outbreak of measles and from clinically suspected sporadic measles patients in 2003. Vero/SLAM cell lines recommended by WHO were used to isolate measles virus. Four hundreds and fifty nucleotides of COOH-terminal of nucleoprotein (N) genes were amplified by using PR-PCR. The amplified products were sequenced and the sequences were compared with references viruses from GeneBank.</p><p><b>RESULTS</b>Eight strains of measles viruses were isolated from throat swabs of patients who came from seven districts and counties of Beijing. Sequence analysis of the 450 nucleotides of COOH-terminal of nucleoprotein (N) genes indicated that these 8 strains belonged to H1a genotype. The average genetic distances of these 8 strains to H1a genotype, Chin9322, H1b genotype, Chin9475 and H1c genotype, Chin9427, were 0.004 - 0.011, 0.026 - 0.031 and 0.015 - 0.022, respectively. The average genetic distances of these 8 strains to H1a genotype, Anhui 01 - 1/Anhui 02 - 2, were 0.000 - 0.009 (0 - 5 nucleotide variation).</p><p><b>CONCLUSIONS</b>Major genotypes of wild-type measles viruses circulated in Beijing in 2003 were H1a genotype. The genotypes H1c, H1b and H2 may have disappeared in Beijing.</p>
Subject(s)
Child , Humans , China , Epidemiology , Disease Outbreaks , Genotype , Immunoglobulin M , Blood , Measles , Epidemiology , Allergy and Immunology , Virology , Measles virus , Classification , Genetics , Allergy and Immunology , Nucleoproteins , Genetics , RNA, Viral , Reverse Transcriptase Polymerase Chain Reaction , Viral Proteins , GeneticsABSTRACT
Objective To explore the characteristics viral pathogens in hospitalized children with lower respiratory infection,and to provide reference data for diagnosis and treatment.Methods Nasopharyngeal secretion(NPS) samples were collected from hospitalized patients with lower respiratory infection(LRI) from Dec.2005 to Apr.2006.The NPS samples were detected for 7 respiratory virus antigens including respiratory syncytial virus(RSV),influenza virus A and B(IVA and IVB),parainfluenza virus 1,2,3(PIV 1,2,3) and adenovirus(ADV) by indirect immunofluorescent assay.Results Nine hundred and thirty-five NPS samples were collected from children(597 boys,338 girls) with LRI.The mean age was 7.5 months(range from 1 day to 6 years).Viral pathogens were identified in 516(55.2%) samples.The positive rate of RSV decreased with increasing of age,whereas the positive rate of IV and PIV increased.ADV was only detected in children less than 3 years of age,accounting for 0.6%-6.2%.Conclusions Viral pathogens are the main etiology of LRI in young children in Beijing area from Dec.2005 to Apr.2006.RSV is the most frequent viral pathogens,followed by IV and PIV.
ABSTRACT
<p><b>BACKGROUND</b>This study was to assess certain epidemiological and clinical characteristics of astrovirus infection in children with diarrhea below 5 years old hospitalized at the Beijing Children's Hospital.</p><p><b>METHODS</b>From September 1999 to August 2001, a total of 288 children with diarrhea below 5 years old hospitalized at Beijing Children's Hospital were studied. Fecal specimens collected from each patient were tested for astrovirus by using enzyme immunoassay (EIA) and further serotyped by reverse-transcription polymerase chain reaction (RT-PCR) using serotype-specific primers.</p><p><b>RESULTS</b>Astrovirus antigen was positive in 9.0% of the patients, and the rate of coinfection with rotavirus was 19.2% among the astrovirus positive patients. The patients with astrovirus infection were at the age of 7 days to 17 months, most (92.3%) of them were less than 12 months. Astrovirus infection occurred mainly between October and March of the following year. Main symptoms included watery stool, vomiting, fever, and dehydration. Increased activity of myocardial enzyme was seen in 4 patients. Of the 22 astrovirus positive specimens, 14 were successfully serotyped by RT-PCR and they were all serotype 1.</p><p><b>CONCLUSIONS</b>The overall incidence of astrovirus infection in young children with diarrhea hospitalized at the Beijing Children's Hospital was 9.0%. The predominant serotype was type 1. Astrovirus may play an important role in viral diarrhea in young children in Beijing, China.</p>